RNA-Seq

We can offer two RNA sequencing service: RNA-Seq and AmpliSeq RNA.

The service includes:

  • sample quantity and quality controls
  • library preparation starting from totalRNA or enriched RNA samples extracted from fresh or FFPE tissues and cells
  • library sequencing with IonProton (Thermo Fisher Scientific) or NextSeq500 (Illumina)
  • bioinformatics analysis: alignment on a reference genome to generate alignment BAM files, count and normalization of reads mapping on each gene. Resulting table with gene expression values
  • data storage: data and results are saved on an hard disk and returned to customer by express delivery

The Ampliseq™ Solution (Life Technologies)

The Ion AmpliSeq™ Transcriptome Human Gene Expression Kit enables the simultaneous measurement of the expression levels of over 20,000 human RefSeq genes in a single assay. Providing the ability to work directly with low input levels of total RNA derived from challenging samples such as formalin-fixed paraffin-embedded (FFPE) tissue or other samples with degraded RNA, the Ion AmpliSeq™ Transcriptome Human Gene Expression Kit is ideal for those interested in obtaining comprehensive, highly sensitive, and easily obtained gene expression measurements from a wide range of RNA sources.

Price: from 250 euro/sample depending on the service (VAT not included)

To request a quotation or place an order, please get to our WEBSITE or contact us at ngs@cribi.unipd.it.