Only Run

NextSeq500 (Illumina)

Library (or pool) have to be quantified by Qubit or RealTime PCR according to the manufacturer’s protocol. We ask:

  • library details (used kit, fragment lenght, index type);
  • 4 nM library pool;
  • at least 15 ul;

Only run options (price/run including reagents):

  • Flow cell High 75 bases 1936 euro 
  • Flow cell High 150 bases 3254 euro
  • Flow cell High 300 bases 4908 euro
  • Flow cell Mid 150 bases 1550 euro
  • Flow cell Mid 300 bases 2184 euro

(VAT not included)

Ion Proton (Life Technologies)

Library (or pool) have to be quantified by Qubit or RealTime PCR according to the manufacturer’s protocol. We require:

  • library details (used kit, fragment lenght, BC name);
  • at least 1 ng of library pool;
  • concentration of library pool not less than 200 pg/ul (Qubit measure);
  • about 10 ul volume of library pool

The service includes:

  • amplification of the library with Ion OneTouch™ 2 Instrument
  • enrichment of the library with Ion OneTouch™ ES Instrument
  • run with Ion Proton Sequencer
  • Bioinformatic analysis as specified for each sequencing service

Price: 1200 euro (VAT not included)

To request a quotation or place an order, please get to our WEBSITE or contact us at ngs@cribi.unipd.it.