NGS Services

For requesting a quotation or place an order, please get to our WEBSITE or contact us at ngs@cribi.unipd.it.

Choose the NGS service you need form the list below and see what we can offer.
Select here for other services.

  1. Whole Exome
  2. Whole Genome
  3. RNA-Seq
  4. miRNA-Seq
  5. Custom Panel
  6. Only Run
  7. Bioinformatics

Custom Panel

Targeted gene panels are useful for sequencing genes of interest to high depth (500–1000× or higher), allowing identification of rare variants.
Pre-designed or Custom panels contain a select set of genes or gene regions that have known or suspected associations with the disease or phenotype under study.

We can offer pre-designed gene panels or support you in your custom panel design.

For any other information please contact us at ngs@cribi.unipd.it.

 

Whole Genome

Whole-genome sequencing is the most comprehensive method for analyzing genomes.
NextSeq500 Illumina sequencer is a scalable and flexible technology and it is equally useful for sequencing any genomes from different species, such as agriculturally important livestock, plants, or disease-related microbes.

Whole Genome Services:
– Small Whole-Genome ReSequencing (for small genomes  ≤ 5 Mb such as genome of bacterium, virus, or other microbe)
– Large Whole-Genome ReSequencing (for large genomes > 5 Mb, such as human, plant, or animal genomes)
– De novo sequencing

If you are interested in doing Whole Genome Sequencing please contact us at ngs@cribi.unipd.it

 

Only Run

NextSeq500 (Illumina)

Library (or pool) have to be quantified by Qubit or RealTime PCR according to the manufacturer’s protocol. We ask:

  • library details (used kit, fragment lenght, index type);
  • 4 nM library pool;
  • at least 15 ul;

Only run options (price/run including reagents):

  • Flow cell High 75 bases 1936 euro 
  • Flow cell High 150 bases 3254 euro
  • Flow cell High 300 bases 4908 euro
  • Flow cell Mid 150 bases 1550 euro
  • Flow cell Mid 300 bases 2184 euro

(VAT not included)

Ion Proton (Life Technologies)

Library (or pool) have to be quantified by Qubit or RealTime PCR according to the manufacturer’s protocol. We require:

  • library details (used kit, fragment lenght, BC name);
  • at least 1 ng of library pool;
  • concentration of library pool not less than 200 pg/ul (Qubit measure);
  • about 10 ul volume of library pool

The service includes:

  • amplification of the library with Ion OneTouch™ 2 Instrument
  • enrichment of the library with Ion OneTouch™ ES Instrument
  • run with Ion Proton Sequencer
  • Bioinformatic analysis as specified for each sequencing service

Price: 1200 euro (VAT not included)

To request a quotation or place an order, please get to our WEBSITE or contact us at ngs@cribi.unipd.it.